Results | viHumans

UniProt ID

Q99IE7

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]

Gene Name

Not Available

Protein Name

♦Non-structural polyprotein p200 (p200) [Cleaved into: Protease p150 (p150) (EC 3.4.22.-)
♦ RNA-directed RNA polymerase/triphosphatase/helicase p90 (p90) (EC 2.7.7.48) (EC 3.6.1.15) (EC 3.6.4.13)]

Organism Name

Rubella Virus (strain TO-336 Vaccine) (RUBV)

Taxonomic Lineage

Viruses> SsRNA Viruses> SsRNA Positive-strand Viruses> No DNA Stage> Togaviridae> Rubivirus> Rubella Virus (RUBV)> Rubella Virus (strain TO-336 Vaccine) (RUBV)

RefSeq

NC_001545.2 ; M15240.2

PubMed ID

11282189

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							MEKLLDEVLAPGGPYNLTVGSWVRDHVRSIVEGAWEVRDVVTAAQKRAIVAVIPRPVFTQMQVSDHPALHAISRYTRRHWIEWGPKEALHVLIDPSPGLL
REVARVERRWVALCLHRTARKLATALAETASEAWHADYVCALRGAPSGPFYVHPEDVPHGGRAVADRCLLYYTPMQMCELMRTIDATLLVAVDLWPVALA
AHVGDDWDDLGIAWHLDHDGGCPADCRGAGAGPTPGYTRPCTTRIYQVLPDTAHPGRLYRCGPRLWTRDCAVAELSWEVAQHCGHQARVRAVRCTLPIRH
VRSLQPSARVRLPDLVHLAEVGRWRWFSLPRPVFQRMLSYCKTLSPDAYYSERVFKFKNALSHSITLAGNVLQEGWKGTCAEEDALCAYVAFRAWQSNAR
LAGIMKSAKRCAADSLSVAGWLDTIWDAIKRFFGSVPLAERMEEWEQDAAVAAFDRGPLEDGGRHLDTVQPPKSPPRPEIAATWIVHAASADRHCACAPR
RDAPRERPSAPAGPPDDEALIPPWLFAERRALRCREWDFEALRARADTAAAPAPLAPRPARCPTVLYRHPAHYGPWLTLDEPGEADAALVLCDPLGQPLR
GPERHFAAGAHMCAQARGLQAFVRVVPPPERPWADGGARAWAKFFRGCAWAQRLLGEPAVMHLPYTDGDVPQLIALALRTLAQQGAALALSVRDLPGGAA
FDANAVTAAVRAGPGQSAATSPPPGDPPPPRRARRSQRHLDARGTPPPAPARDPPPPAPSPPAPPRAGDPVLPTSAGPADRARHAELEVAYEPSDPPTPT
KADPDSDIVESYARAAGPVHLRVRDIMDPPPGCKVVVNAANEGLLAGSGVCGAIFANATAALAADCRRLAPCPTGEAVATPGHGCGYTHIIHAVAPRRPR
DPAALEEGEALLERAYRSIVALAAARRWACVACPLLGAGVYGWSAAESLRAALAATRAEPAERVSLHICHPDRATLTHASVLVGAGLAARRVSPPPTEPL
ASCPAGDPGRPAQRSASPPATPLGDATAPEPRGCQGCELCRYTRVTNDRAYVNLWLERDRGATSWAMRIPEVVVYGPEHLATHFPLNHYSVLKPAEVRPP
RGMCGSDMWRCRGWQGMPQVRCTPSNAHAALCRTGVPPRVSTRGGELDPNTCWLRAAASVAQAARACGAYTSAGCPKCAYGRALSEARTHEDFAALSQRW
SASHADASPDGTGDPLDPLMETVGCACSRVWVGSEHEAPPDHLLVSLHRAPNGPWGVVLEVRARPEGGNPTGHFVCAVGGGPRRVSDRPHLWLAVPLSRG
GGTCAATDEGLAQAYYDDLEVRRLGDDAMARAALASVQRPRKGPYNIRVWDMAAGAGKTTRILAAFTREDLYVCPTNALLHEIQAKLRARDIDIKNAATY
ERALTKPLAAYRRIYIDEAFTLGGEYCAFVASQTTAEVICVGDRDQCGPHYANNCRTPVPDRWPTERSRHTWRFPDCWAARLRAGLDYDIEGERTGTFAC
NLWDGRQVDLHLAFSRETVRRLHEAGIRAYTVREAQGMSVGTACIHVGRDGTDVALALTRDLAIVSLTRASDALYLHELEDGSLRAAGLSAFLDAGALAE
LKEVPAGIDRVVAVEQAPPPLPPADGIPEAQDVPPFCPRTLEELVFGRAGHPHYADLNRVTEGEREVRYMRISRHLLNKNHTEMPGTERVLSAVCAVRRY
RAGEDGSTLRTAVARQHPRPFRQIPPPRVTAGVAQEWRMTYLRERIDLTDVYTQMGVAARELTDRYARRYPEIFAGMCTAQSLSVPAFLKATLKCVDAAL
GPRDTEDCHAAQGKAGLEIRAWAKEWVQVMSPHFRAIQKIIMRALRPQFLVAAGHTEPEVDAWWQAHYTTNAIEVDFTEFDMNQTLATRDVELEISAALL
GLPCAEDYRALRAGSYCTLRELGSTETGCERTSGEPATLLHNTTVAMCMAMRMVPKGVRWAGIFQGDDMVIFLPEGARSAALKWTPAEVGLFGFHIPVKH
VSTPTPSFCGHVGTAAGLFHDVMHQAIKVLCRRFDPDVLEEQQVALLDRLRGVYAALPDTVAANAAYYDYSAERVLAIVRELTAYARGRGLDHPATIGAL
EEIQTPYARANLHDAD
						

Length

2116

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-06-2001

Protein Existence

Inferred from homology

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

♦Non-structural polyprotein p200 replicates the 40S (+) genomic RNA into (-) antigenomic RNA. It cannot replicate the (-) into (+) until cleaved in p150 and p90 (By similarity).
♦ Protease p150 has 2 separate domains with different biological activities. The N-terminal section has presumably a cytoplasmic mRNA-capping activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The C-terminal section harbors a protease active in cis or in trans which specifically cleaves and releases the two mature proteins (By similarity).
♦ RNA-directed RNA polymerase/triphosphatase/helicase p90 replicates the 40S genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This 24S mRNA codes for structural proteins (By similarity).

EC

3.4.22.- , 2.7.7.48 , 3.6.1.15 , 3.6.4.13

Experimentally known GO terms

GO:0003723 ;   GO:0003968 ;   GO:0004197 ;   GO:0004386 ;   GO:0005524 ;
GO:0006351 ;   GO:0006396 ;   GO:0008174 ;   GO:0016020 ;   GO:0039694 ;
GO:0044161 ;   GO:0044175 ;   GO:0044188 ;   GO:0046872

Subcellular Location

♦ Non-structural polyprotein p200: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host cytoplasmic vesicle . Note=Located in vesicles at the cytoplasmic surface of modified endosomes and lysosomes, also called replication complexes. .
♦ Protease p150: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host cytoplasmic vesicle . Note=Located in vesicles at the cytoplasmic surface of modified endosomes and lysosomes, also called replication complexes. .
♦ RNA-directed RNA polymerase/triphosphatase/helicase p90: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host cytoplasmic vesicle . Note=Located in vesicles at the cytoplasmic surface of modified endosomes and lysosomes, also called replication complexes. .

Domain(s)

♦DOMAIN 57 247 Alphavirus-like MT.
♦ DOMAIN 806 985 Macro.
♦ DOMAIN 1109 1274 Peptidase C27.
♦ DOMAIN 1320 1468 (+)RNA virus helicase ATP-binding.
♦ DOMAIN 1469 1609 (+)RNA virus helicase C-terminal.
♦ DOMAIN 1870 1981 RdRp catalytic.

Motif(s)

MOTIF 1902 1906 Human RB1 binding motif.

Methods of 3D structure generation

Predicted/Modelled

Experimetally known structure

Not Available

Active Site(s)

♦ACT_SITE 1152 1152 For cysteine protease activity.
♦ ACT_SITE 1273 1273 For cysteine protease activity.

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available