Results | viHumans

UniProt ID

Q66578

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]; Macaca (macaques) [TaxID: 9539]

Gene Name

Not Available

Protein Name

♦Genome polyprotein [Cleaved into: Protein VP0 (P1AB) (Virion protein 0)
♦ Protein VP3 (P1C) (Virion protein 3)
♦ Protein VP1 (P1D) (Virion protein 1)
♦ Protein 2A (P2A)
♦ Protein 2B (P2B)
♦ Protein 2C (P2C) (EC 3.6.1.15)
♦ Protein 3A (P3A)
♦ Protein 3B (P3B) (VPg)
♦ Protease 3C (P3C) (EC 3.4.22.28) (EC 3.4.22.29) (Picornain 3C)
♦ RNA-directed RNA polymerase 3D-POL (P3D-POL) (EC 2.7.7.48)]

Organism Name

Human Parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22)

Taxonomic Lineage

Viruses> SsRNA Viruses> SsRNA Positive-strand Viruses> No DNA Stage> Picornavirales> Picornaviridae> Parechovirus> Parechovirus A> Human Parechovirus 1> Human Parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22)

RefSeq

NC_038319.1 ; S45208.1

PubMed ID

1528901 ; 7966616 ; 11160695

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							METIKSIADMATGVVSSVDSTINAVNEKVESVGNEIGGNLLTKVADDASNILGPNCFATTAEPENKNVVQATTTVNTTNLTQHPSAPTMPFSPDFSNVDN
FHSMAYDITTGDKNPSKLVRLETHEWTPSWARGYQITHVELPKVFWDHQDKPAYGQSRYFAAVRCGFHFQVQVNVNQGTAGSALVVYEPKPVVTYDSKLE
FGAFTNLPHVLMNLAETTQADLCIPYVADTNYVKTDSSDLGQLKVYVWTPLSIPTGSANQVDVTILGSLLQLDFQNPRVFAQDVNIYDNAPNGKKKNWKK
IMTMSTKYKWTRTKIDIAEGPGSMNMANVLCTTGAQSVALVGERAFYDPRTAGSKSRFDDLVKIAQLFSVMADSTTPSENHGVDAKGYFKWSATTAPQSI
VHRNIVYLRLFPNLNVFVNSYSYFRGSLVLRLSVYASTFNRGRLRMGFFPNATTDSTSTLDNAIYTICDIGSDNSFEITIPYSFSTWMRKTNGHPIGLFQ
IEVLNRLTYNSSSPSEVYCIVQGKMGQDARFFCPTGSVVTFQNSWGSQMDLTDPLCIEDDTENCKQTMSPNELGLTSAQDDGPLGQEKPNYFLNFRSMNV
DIFTVSHTKVDNLFGRAWFFMEHTFTNEGQWRVPLEFPKQGHGSLSLLFAYFTGELNIHVLFLSERGFLRVAHTYDTSNDRVNFLSSNGVITVPAGEQMT
LSAPYYSNKPLRTVRDNNSLGYLMCKPFLTGTSTGKIEVYLSLRCPNFFFPLPAPKVTSSRALRGDMANLTNQSPYGQQPQNRMMKLAYLDRGFYKHYGI
IVGDHVYQLDSDDIFKTALTGKAKFTKTKLTSDWVIEEECELDYFRIKYLESAVDSEHIFSVDKNCETIAKDIFGTHTLSQHQAIGLVGTILLTAGLMST
IKTPVNAVTIKEFFNHAIDGDEQGLSLLVQKCTTFFSSAATEILDNDLVKFIVKILVRILCYMVLYCHKPNILTTACLSTLLIMDVTSSSVLSPSCKALM
QCLMDGDVKKLAEIVAESMSNTDDDEVKEQICDTVKYTKTILSNQGPFKGFNEVSTAFRHIDWWIHTLLKIKDMVLSVFKPSIESKAIQWLERNKEHVCS
ILDYASDIIVESKDQSKMKTQDFYQRYSDCLAKFKPIMAICFRSCHNSISNTVYRLFQELARIPNRISTNNDLIRIEPIGIWIQGEPGQGKSFLTHTLSR
QLQKSCKLNGVFTNPTASEFMDGYDNQDIHLIDDLGQTRKEKDIEMLCNCISSVPFIVPMAHLEEKGKFYTSKLVVATTNKSDFSSTVLQDSGALKRRFP
YIMHIRAAKAYSKAGKLNVSQAMATMSTGECWEVSKNGRDWETLKLKDLVDKITIDYNERVKNYNAWKQQLENQTLDDLDDAVSYIKHNFPDAIPYVDEY
LNIEMSTLIEQMEAFIEPKPSVFKCFANKIGSKISKASREVVDWFSDKIKSMLSFVERNKAWLTVVSAVTSAISILLLVTKIFKKEESKDERAYNPTLPV
AKPKGTFPVSQREFKNEAPYDGQLEHIISQMAYITGSTTGHMTHCAGYQHDEIILHGHSIKYLEQEDELTLHYKNKVFPIEQPSVTQVTLGGKPMDLAIL
KCKLPFRFKKNSKYYTNKIGTESMLIWMTEQGIITKEVQRVHHSGGIKTREGTESTKTISYTVKSCKGMCGGLLISKVEGNFKILGMHIAGNGEMGVAIP
FNFLKNDMSDQGIVTEITPIQPMYINTKTQIHKSPVYGAVEVKMGPAVLSKSDTRLEEPVECLIKKSASKYRVNKFQVNNELWQGVKACVKSKFREIFGM
NGIVDMKTAILGTSHVNSMDLSTSAGYSFVKSGYKKKDLICLEPFSVAPLLERLVQDKFHNLLKGNQITTTFNTCLKDELRKLDKIASGKTRCIEACEVD
YCIVYRMIMMEIYDKIYQTPCYYSGLAVGINPYKDWHFMINALNDYNYEMDYSQYDGSLSSMLLWEAVEVLAYCHDSPDLVMQLHKPVIDSDHVVFNERW
LIHGGMPSGSPCTTVLNSLCNLMMCIYTTNLISPGIDCLPIVYGDDVILSLDKEIEPEKLQSIMADSFGAEVTGSRKDEPPSLKPRMEVEFLKRKPGYFP
ESTFIVGKLDTENMIQHLMWMKNFSTFKQQLQSYLMELCLHGKDTYQHYIKILEPYLQEWNITVDDYDVVITKLMPMVFD
						

Length

2180

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-11-1996

Protein Existence

Evidence at protein level

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

♦Capsid proteins VP0, VP2, VP3 form a closed capsid enclosing the viral positive strand RNA genome. Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis.
♦ Protein 2A: Is not a protease.
♦ Protein 2B: Affects membrane integrity and cause an increase in membrane permeability.
♦ Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).
♦ Protein 3A, via its hydrophobic domain, serves as membrane anchor.
♦ Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease (By similarity).
♦ RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals.

EC

3.6.1.15 , 3.4.22.28 , 3.4.22.29 , 2.7.7.48

Experimentally known GO terms

GO:0003723 ;   GO:0003724 ;   GO:0003968 ;   GO:0004197 ;   GO:0005198 ;
GO:0005216 ;   GO:0005524 ;   GO:0006351 ;   GO:0016020 ;   GO:0018144 ;
GO:0019015 ;   GO:0019028 ;   GO:0019062 ;   GO:0039520 ;   GO:0039611 ;
GO:0039694 ;   GO:0039707 ;   GO:0044162 ;   GO:0044385 ;   GO:0051259 ;
GO:0075512

Subcellular Location

♦ Protein VP3: Virion . Host cytoplasm .
♦ Protein VP1: Virion . Host cytoplasm .
♦ Protein 2B: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 2C: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 3A: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 3B: Virion .
♦ Protease 3C: Host cytoplasm .
♦ RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .

Domain(s)

♦DOMAIN 1160 1318 SF3 helicase.
♦ DOMAIN 1945 2059 RdRp catalytic.

Motif(s)

MOTIF 764 766 Cell attachment site.

Methods of 3D structure generation

X-ray crystallography (2); Electron microscopy (1)

Experimetally known structure

4UDF 4Z92 5MJV

Active Site(s)

♦ACT_SITE 1550 1550 For protease 3C activity.
♦ ACT_SITE 1581 1581 For protease 3C activity.
♦ ACT_SITE 1670 1670 For protease 3C activity.

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available