Reviewed
Gerbillinae (gerbils) [TaxID: 10045]; Homo Sapiens (Human) [TaxID: 9606]; Phlebotomus Papatasi (Sandfly) [TaxID: 29031]
L[Gene ID: 14857914 ]
♦RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) [Includes: RNA-directed RNA polymerase (EC 2.7.7.48)
♦ mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56)
♦ GDP polyribonucleotidyltransferase (EC 2.7.7.88)
♦ Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC 2.1.1.296)]
♦ mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56)
♦ GDP polyribonucleotidyltransferase (EC 2.7.7.88)
♦ Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC 2.1.1.296)]
Isfahan Virus (ISFV)
Viruses> SsRNA Viruses> SsRNA Negative-strand Viruses> Mononegavirales> Rhabdoviridae> Vesiculovirus> Isfahan Vesiculovirus> Isfahan Virus (ISFV)
Not Available
Various pathway(s) in which protein is involved
Not Available
MDEYSEEKWGDSDEESFGTGKYSDESRIRGLNSVDYNLNSPLIQDDLYYLMERVRGRPVPPIWKAKNWTETIHLVQESRLDYLPTQKLHSWYAEWLMEES
HDSSQGLAFLKEVDKDSLETYEVVMSFLRGWCGGAPAYKKKEGRHIAKIGSLCQKFLDLHRVILIMNASTQMELSNLAETFQASSVSKKIITTPSMGKME
MSGQFALAYQQKVILDRNFLLMMKDVVIGRMQTLLSMVSRTDDKFSDGDISYLIKIYQLGDKIIQSLGNDGYELIKTIEPMCNLRLSDLAREYRPLIPEF
PHFRQHIEGTVSELRKKTALIVDMFKMIDRTPGVDITLVIYGSFRHWGHPFIDYFAGLTKLNSQVTMGKQIDDEYVACLASDLARIVLTKEFNEKKRWSV
NYNLVPQDHPFHEHIRDNTWPTPAVIQDFGDKWHELPLTQCFEIPDLIDPSIIYSDKSHSMNRQDVLNHVKRKPDQPIPSRKVLQTMIDTPATNWLEFLE
EIDKNGLSDDDLVIGLKGKERELKIAGRFFSLMSWKLREYFVITEYLIKTHFVPLFHGLTMADDMTAVIKKMLESSSGQGLKDYSAVCIANHIDYEKWNN
HQRKRSNEPIFKVMGQFLGFPNLISRTHEFFEKSLIYYNGRPDLMKVQDGRLVNTTKQLVCWEGQAGGLEGLRQKGWSILNLLVIQRESKIRNTAVKVLA
QGDNQVICTQYKTKQHRNETELRSALTQMKLNNDAVMKAIESGTNKLGLLINQDETMQSADYLNYGKVPIFRGVIRGLETKRWSRVTCVTNDQLPTCANL
MSSVSTNALTVAHFDVTPLNAMIQYNYFGNFSRLLLNMHDPAVRCSLFQLSQKHKIDLFSFEFKVGVLYLDPSIGGVCGTALSRFLIRGFPDPVTESISF
WKVIYNNTQDNRLKKLCTAFGNPKIAQFRYSHIEKLLEDPTSLNISMGMSAANLLKSEIKKNLLRKRRTIGNSIVRDAVTYIHSEDEKIRSYLWSINPLF
PRFLSEFKSGTFMGVASSVVSLFQNSRTIRNVFKDYMSSAIDELITKSEVNSLEHLCKYKGVRNFDQVWKCSASQADYLRRLSWGRKVLGTTIPHPLEML
GAGTIKNNSSTCCEHSGQDYISVFCPKGISNVLIERGPMAAYLGSKTSESTSILQPWEKESKIPIIKRATRLRDAIHWFVEPSSNLAKSILQNITALTGE
EWGSSLEGFKRTGSALHRFTTSRMSHGGFCAQSPAALTRMMATTDTMSDYAKDNYDFMFQACLLFSQITTSVLLLETTISNTVHFHTRCINCVRKIEEPW
LESPSVLQSKDVSNVLASWRNGGGSWGEQLHQLKPLKGDWEILTPAEKSYHVGRTLGFLFGDLTGQSSIRADDSSLFPLSIQKRLRGRGFLRGVLDGLVR
ASACQVIHRRSLTQLKRPANAVYGGLIFLIDKISASSTFINLCRDGPIREELSSIPHKIPTSYPTSNADLGLHIRNYFKFQCKSVELGKYQSDLEDLWLF
SDLLSSGFAGPYALSSKVLKSLYKPSLSRRDRNNIRKLGALSRLLRSHENWSELHKEFLTSQLLLCQEEVRHACKFGIPKNVSAKSSMVWGKEAVSYVLD
IPVEFTSQKQTKHLNACPRIQDPTISGLRLGQLPTGAHYKIRTILNAYNIKCRDVLCGGDGSGGMTAACLRYYSNSRAIFNSILEFDGSSMKGSSPDPPS
ALETVDQGMVRCVNATTCWENPSDLSQERTWDYFLHLKKSFNMKIDLIILDMEVRDFQISKLIEGNLRLKISKLLEKNGTLIYKTYGTIICSETSNVLTT
LGPLFHSVYIVQTGYSSSFTSEVYVLFSKQKSFVDSPYVDWGSLQYNWEKLACFRNPRQEFKRALRIRSSRSLMGIPSSFLPDPLVNLETLLQISGVPSG
VSHQLVTDVKSSGASGLSSAIGLLGLISHFTLDVTKLYVQEYRPPSDNRLIKMASAITGISYWISIAYHDQQLNQALTSVIKKSFPIRWGLINHRLHWSV
SDRFHRSKDVRLSDCLAGIGNWIRGMELMKLPAGMFSHKEVNMILSKYIRGLNYHTISSRTGILEILKSQFSIIDRSLMTITTDNIQSSDWTD
HDSSQGLAFLKEVDKDSLETYEVVMSFLRGWCGGAPAYKKKEGRHIAKIGSLCQKFLDLHRVILIMNASTQMELSNLAETFQASSVSKKIITTPSMGKME
MSGQFALAYQQKVILDRNFLLMMKDVVIGRMQTLLSMVSRTDDKFSDGDISYLIKIYQLGDKIIQSLGNDGYELIKTIEPMCNLRLSDLAREYRPLIPEF
PHFRQHIEGTVSELRKKTALIVDMFKMIDRTPGVDITLVIYGSFRHWGHPFIDYFAGLTKLNSQVTMGKQIDDEYVACLASDLARIVLTKEFNEKKRWSV
NYNLVPQDHPFHEHIRDNTWPTPAVIQDFGDKWHELPLTQCFEIPDLIDPSIIYSDKSHSMNRQDVLNHVKRKPDQPIPSRKVLQTMIDTPATNWLEFLE
EIDKNGLSDDDLVIGLKGKERELKIAGRFFSLMSWKLREYFVITEYLIKTHFVPLFHGLTMADDMTAVIKKMLESSSGQGLKDYSAVCIANHIDYEKWNN
HQRKRSNEPIFKVMGQFLGFPNLISRTHEFFEKSLIYYNGRPDLMKVQDGRLVNTTKQLVCWEGQAGGLEGLRQKGWSILNLLVIQRESKIRNTAVKVLA
QGDNQVICTQYKTKQHRNETELRSALTQMKLNNDAVMKAIESGTNKLGLLINQDETMQSADYLNYGKVPIFRGVIRGLETKRWSRVTCVTNDQLPTCANL
MSSVSTNALTVAHFDVTPLNAMIQYNYFGNFSRLLLNMHDPAVRCSLFQLSQKHKIDLFSFEFKVGVLYLDPSIGGVCGTALSRFLIRGFPDPVTESISF
WKVIYNNTQDNRLKKLCTAFGNPKIAQFRYSHIEKLLEDPTSLNISMGMSAANLLKSEIKKNLLRKRRTIGNSIVRDAVTYIHSEDEKIRSYLWSINPLF
PRFLSEFKSGTFMGVASSVVSLFQNSRTIRNVFKDYMSSAIDELITKSEVNSLEHLCKYKGVRNFDQVWKCSASQADYLRRLSWGRKVLGTTIPHPLEML
GAGTIKNNSSTCCEHSGQDYISVFCPKGISNVLIERGPMAAYLGSKTSESTSILQPWEKESKIPIIKRATRLRDAIHWFVEPSSNLAKSILQNITALTGE
EWGSSLEGFKRTGSALHRFTTSRMSHGGFCAQSPAALTRMMATTDTMSDYAKDNYDFMFQACLLFSQITTSVLLLETTISNTVHFHTRCINCVRKIEEPW
LESPSVLQSKDVSNVLASWRNGGGSWGEQLHQLKPLKGDWEILTPAEKSYHVGRTLGFLFGDLTGQSSIRADDSSLFPLSIQKRLRGRGFLRGVLDGLVR
ASACQVIHRRSLTQLKRPANAVYGGLIFLIDKISASSTFINLCRDGPIREELSSIPHKIPTSYPTSNADLGLHIRNYFKFQCKSVELGKYQSDLEDLWLF
SDLLSSGFAGPYALSSKVLKSLYKPSLSRRDRNNIRKLGALSRLLRSHENWSELHKEFLTSQLLLCQEEVRHACKFGIPKNVSAKSSMVWGKEAVSYVLD
IPVEFTSQKQTKHLNACPRIQDPTISGLRLGQLPTGAHYKIRTILNAYNIKCRDVLCGGDGSGGMTAACLRYYSNSRAIFNSILEFDGSSMKGSSPDPPS
ALETVDQGMVRCVNATTCWENPSDLSQERTWDYFLHLKKSFNMKIDLIILDMEVRDFQISKLIEGNLRLKISKLLEKNGTLIYKTYGTIICSETSNVLTT
LGPLFHSVYIVQTGYSSSFTSEVYVLFSKQKSFVDSPYVDWGSLQYNWEKLACFRNPRQEFKRALRIRSSRSLMGIPSSFLPDPLVNLETLLQISGVPSG
VSHQLVTDVKSSGASGLSSAIGLLGLISHFTLDVTKLYVQEYRPPSDNRLIKMASAITGISYWISIAYHDQQLNQALTSVIKKSFPIRWGLINHRLHWSV
SDRFHRSKDVRLSDCLAGIGNWIRGMELMKLPAGMFSHKEVNMILSKYIRGLNYHTISSRTGILEILKSQFSIIDRSLMTITTDNIQSSDWTD
2093
Not Available
Not Available
15-02-2005
Inferred from homology
| Amino Acid | Count | % Frequency | Amino Acid | Count | % Frequency |
|---|---|---|---|---|---|
| Alanine (A) | Leucine (L) | ||||
| Arginine (R) | Lysine (K) | ||||
| Asparagine (N) | Methionine (M) | ||||
| Aspartic Acid (D) | Phenylalanine (F) | ||||
| Cysteine (C) | Proline (P) | ||||
| Glutamine (Q) | Serine (S) | ||||
| Glutamic Acid (E) | Threonine (T) | ||||
| Glycine (G) | Tryptophan (W) | ||||
| Histidine (H) | Tyrosine (Y) | ||||
| Isoleucine (I) | Valine (V) |
| % Number of Residues in Helices | % Number of Residues in Strands | % Number of Residues in Coils |
|---|---|---|
♦RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
♦ RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
♦ RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
2.7.7.48 , 2.1.1.56 , 2.7.7.88 , 2.1.1.296
Virion . Host cytoplasm . Note=L and P are packaged asymmetrically towards the blunt end of the virus. .
♦DOMAIN 587 773 RdRp catalytic.
♦ DOMAIN 1629 1826 Mononegavirus-type SAM-dependent 2'-O-MTase.
♦ DOMAIN 1629 1826 Mononegavirus-type SAM-dependent 2'-O-MTase.
Not Available
Predicted/Modelled
Not Available
Not Available
Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.
Not Available
- Million Molecules
Best 20 Hit molecules
Not Available