Results | viHumans

UniProt ID

P41266

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]; Myodes Glareolus (Bank Vole) (Clethrionomys Glareolus) [TaxID: 447135]

Gene Name

GP

Protein Name

♦Envelopment polyprotein (M polyprotein) [Cleaved into: Glycoprotein N (Gn) (Glycoprotein G1)
♦ Glycoprotein C (Gc) (Glycoprotein G2)]

Organism Name

Puumala Virus (strain P360)

Taxonomic Lineage

Viruses> SsRNA Viruses> SsRNA Negative-strand Viruses> Bunyavirales> Hantaviridae> Orthohantavirus> Puumala Orthohantavirus> Puumala Virus (strain P360)

RefSeq

NC_005225.1 ; X61034.1 ; NC_005223.1 ; X61034.1 ; NC_005224.1 ; Z66548.1

PubMed ID

8266723

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							MGELSPVCLCLLLQGLLLCNTGAARNLNELKMECPHTIRLGQGLVVGSVELPSLPIQQVETLKLESSCNFDLHTSTAGQQSFTKWTWEIKGDLAENTQAS
STSFQTKSSEVNLRGLCLIPTLVVETAARMRKTIACYDLSCNQTVCQPTVYLMGPIQTCITTKSCLLSLGDQRIQVNYEKTYCVSGQLVEGICFNPIHTM
ALSQPSHTYDIMTMMVRCFLVIKKVTSGDSMKIEKNFETLVQKNGCTANNFQGYYICLIGSSSEPLYVPALDDYRSAEVLSRMAFAPHGEDHDIEKNAVS
AMRIAGKVTGKAPSTESSDTVQGIAFSGSPLYTSTGVLTSKDDPVYIWAPGIIMEGNHSICEKKTLPLTWTGFISLPGEIEKTTQCTVFCTLAGPGADCE
AYSETGIFNISSPTCLINRVQRFRGSEQQIKFVCQRVDMDITVYCNGMKKVILTKTLVIGQCIYTFTSIFSLIPGVAHSLAVELCVPGLHGWATMLLLLT
FCFGWVLIPTITMILLKILIAFAYLCSKYNTDSKFRILIEKVKREYQKTMGSMVCEVCQYECETAKELESHRKSCSIGSCPYCLNPSEATTSALQAHFKV
CKLTSRFQENLRKSLTVYEPMQGCYRTLSLFRYRSRFFVGLVWCVLLVLELIVWAASAETQNLNAGWTDTAHGSGIIPMKTDLELDFSLPSSASYTYRRQ
LQNPANEQEKIPFHLQLSKQVIHAEIQHLGHWMDATFNLKTAFHCYGSCEKYAYPWQTAGCFIEKDYEYETGWGCNPPDCPGVGTGCTACGVYLDKLKSV
GKVFKIVSLRYTRKVCIQLGTEQTCKTVDSNDCLITTSVKVCLIGTISKFQPSDTLLFLGPLQQGGLIFKQWCTTTCQFGDPGDIMSTPTGMKCPELNGS
FRKKCAFATTPVCQFDGNTISGYKRMIATKDSFQSFNVTEPHISTSALEWIDPDSSLRDHINVIVSRDLSFQDLSETPCQIDLATASIDGAWGSGVGFNL
VCTVSLTECSAFLTSIKACDAAMCYGSTTANLVRGQNTIHIVGKGGHSGSKFMCCHDTKCSSTGLVAAAPHLDRVTGYNQADSDKIFDDGAPECGMSCWF
KKSGEWILGVLNGNWMVVAVLVVLLILSILLFTLCCPRRPSYRKEHKP
						

Length

1148

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-02-1995

Protein Existence

Evidence at protein level

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

Glycoprotein N and Glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).

EC

Not Available

Experimentally known GO terms

GO:0007165 ; GO:0016021 ; GO:0019062 ; GO:0039503 ; GO:0039547 ;
GO:0039654 ; GO:0044167 ; GO:0044178 ; GO:0055036

Subcellular Location

♦ Glycoprotein N: Virion membrane . Host Golgi apparatus membrane
♦ Single-pass type I membrane protein . Host endoplasmic reticulum membrane
♦ Single-pass type I membrane protein . Note=Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein N to the Golgi complex, where virion budding occurs. .
♦ Glycoprotein C: Virion membrane . Host Golgi apparatus membrane
♦ Single-pass type I membrane protein .

Domain(s)

DOMAIN 621 644 ITAM.

Motif(s)

Not Available

Methods of 3D structure generation

X-ray crystallography (2)

Experimetally known structure

5J81 5J9H

Active Site(s)

Not Available

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available