Results | viHumans

UniProt ID

P21033

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]

Gene Name

PAPS J3R

Protein Name

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) (Poly(A) polymerase regulatory subunit) (Poly(A) polymerase small subunit) (PAP-S) (VP39)

Organism Name

Vaccinia Virus (strain Copenhagen) (VACV)

Taxonomic Lineage

Viruses> DsDNA Viruses> No RNA Stage> Poxviridae> Chordopoxvirinae> Orthopoxvirus> Vaccinia Virus> Vaccinia Virus (strain Copenhagen) (VACV)

RefSeq

NC_006998.1 ; AY243312.1

PubMed ID

2219722 ;

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							MDVVSLDKPFMYFEEIDNELDYEPESANEVAKKLPYQGQLKLLLGELFFLSKLQRHGILDGATVVYIGSAPGTHIRYLRDHFYNLGMIIKWMLIDGRHHD
PILNGLRDVTLVTRFVDEEYLRSIKKQLHPSKIILISDVRSKRGGNEPSTADLLSNYALQNVMISILNPVASSLKWRCPFPDQWIKDFYIPHGNKMLQPF
APSYSAEMRLLSIYTGENMRLTRVTKSDVVNYEKKMYYLNKIVRNKVVVNFDYPNQEYDYFHMYFMLRTVYCNKTFPTTKAKVLFLQQSIFRFLNIPTTS
TEKVSHEPIQRKISSKNSMSKNRNSKRSVRGNK
						

Length

333

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-02-1991

Protein Existence

Inferred from homology

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.

EC

2.1.1.57

Experimentally known GO terms

GO:0003746 ; GO:0004483 ; GO:0006351 ; GO:0006370 ; GO:0019012 ;
GO:0031440

Subcellular Location

Virion . Note=Localizes to the virion core. .

Domain(s)

Not Available

Motif(s)

Not Available

Methods of 3D structure generation

Predicted/Modelled

Experimetally known structure

Not Available

Active Site(s)

ACT_SITE 175 175 For methyltransferase activity.

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available