Results | viHumans

UniProt ID

P13888

Status

Reviewed

Host

Aedes [TaxID: 7158]; Culex Annulirostris (Common Banded Mosquito) [TaxID: 162997]; Homo Sapiens (Human) [TaxID: 9606]; Macropus Sp. (kangaroo) [TaxID: 9322]

Gene Name

Not Available

Protein Name

♦Non-structural polyprotein (Polyprotein nsP1234) (P1234) [Cleaved into: Non-structural protein 3 (nsP3)
♦ RNA-directed RNA polymerase nsP4 (EC 2.7.7.48) (Non-structural protein 4) (nsP4)] (Fragment)

Organism Name

Ross River Virus (strain T48) (RRV)

Taxonomic Lineage

Viruses> SsRNA Viruses> SsRNA Positive-strand Viruses> No DNA Stage> Togaviridae> Alphavirus (arboviruses Group A)> Ross River Virus> Ross River Virus (strain T48) (RRV)

RefSeq

Not Available

PubMed ID

2834873 ; 6291034

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							APSYRVRRTDISGHAEEAVVNAANAKGTVGDGVCRAVARKWPDSFKGAATPVGTAKLVQANGMNVIHAVGPNFSTVTEAEGDRELAAAYRAVAGIINASN
IKSVAIPLLSTGVFSGGKDRVMQSLNHLFTAMDTTDADVVIYCRDKAWEKKIQEAIDRRTAVELVSEDISLESDLIRVHPDSCLVGRKGYSITDGKLHSY
LEGTRFHQTAVDMAEISTLWPKLQDANEQICLYALGESMDSIRTKCPVEDADSSTPPKTVPCLCRYAMTAERVARLRMNNTKAIIVCSSFPLPKYRIEGV
QKVKCDRVLIFDQTVPSLVSPRKYIPAAASTHADTVSLDSTVSTGSAWSFPSEATYETMEVVAEVHHSEPPVPPPRRRRAQVTMHHQELLEVSDMHTPIA
ARVEIPVYDTAVVVERVAIPCTSEYAKPIPAPRAARVVPVPAPRIQRASTYRVSPTPTPRVLRASVCSVTTSAGVEFPWAPEDLEVLTEPVHCKMREPVE
LPWEPEDVDIQFGDFETSDKIQFGDIDFDQFLGRAGAYIFSSDTGPGHLQQKSVRQHALPCEMLYVHEEERTYPPALDEAREKLLQAKMQMAPTEANKSR
YQSRKVENMKAVIIDRLKDGARTYLTEQSEKIPTYVSKYPRPVYSPSVEDSLQNPEVAVAACNAFLEANYPTVASYQITDEYDAYLDMVDGSESCLDRAT
FCPAKLRCYPKHHAYHQPQVRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVLNVECFKKFACNGEYWQEFKDNPIRITTENITTYVTRLKGP
KAAALFAKTHNLVPLQEVPMDRFVVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLKAVLAPNIHTLFDMSAEDFDAIIAAHFQP
GDAVLETDIASFDKSQDDSLALTALMLLEDLGVDQELLDLIEAAFGEITSVHLPTGTRFKFGAMMKSGMFLTLFVNTLLNIVIACRVLREKLTNSVCAAF
IGDDNIVHGVRSDPLMAERCASWVNMEVKIIDATMCEKPPYFCGGFILYDKVTGSACRVADPLKRLFKLGKPLPAGDTQDEDRRRALKDETDRWARVGLK
SELEIALSSRYEVNGTGNIVRAMATLAKSLKNFKKLRGPIVHLYGGPK
						

Length

1148

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-01-1990

Protein Existence

Evidence at transcript level

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

♦nsP3 is essential for minus strand and subgenomic 26S mRNA synthesis.
♦ nsP4 is an RNA dependent RNA polymerase. It replicates genomic and antigenomic RNA by recognizing replications specific signals. Transcribes also a 26S subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This 26S mRNA codes for structural proteins (By similarity).

EC

2.7.7.48

Experimentally known GO terms

GO:0000166 ; GO:0003723 ; GO:0003968 ; GO:0006351 ; GO:0016020 ;
GO:0039694 ; GO:0044175 ; GO:0044188

Subcellular Location

♦ Non-structural polyprotein: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Located on the cytoplasmic surface of modified endosomes and lysosomes, also called cytopathic vacuoles type I (CPVI). These vacuoles contain numerous small circular invaginations (spherules) which may be the sites of RNA synthesis.
♦ Non-structural protein 3: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host cytoplasm . Note=In the late phase of infection, the polyprotein is quickly cleaved before localization to cellular membranes. Then nsP3 and nsP3' seems to aggregate in cytoplasm (By similarity). .
♦ RNA-directed RNA polymerase nsP4: Host endosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Host lysosome membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side .

Domain(s)

♦DOMAIN <1 160 Macro.
♦ DOMAIN 902 1017 RdRp catalytic.

Motif(s)

Not Available

Methods of 3D structure generation

Predicted/Modelled

Experimetally known structure

Not Available

Active Site(s)

Not Available

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available