♦Genome polyprotein [Cleaved into: Leader protein
♦ Protein VP0 (VP4-VP2)
♦ Protein VP4 (P1A) (Rho) (Virion protein 4)
♦ Protein VP2 (Beta) (P1B) (Virion protein 2)
♦ Protein VP3 (Gamma) (P1C) (Virion protein 3)
♦ Protein VP1 (Alpha) (P1D) (Virion protein 1)
♦ Protein 2A (P2A) (G)
♦ Protein 2B (I) (P2B)
♦ Protein 2C (C) (P2C) (EC 3.6.1.15)
♦ Protein 3A (P3A)
♦ Protein 3B (P3B) (H) (VPg)
♦ Protease 3C (P3C) (EC 3.4.22.28) (Picornain 3C) (p22)
♦ RNA-directed RNA polymerase 3D-POL (E) (P3D-POL) (EC 2.7.7.48)]

NC_001479.1 ;  M81861.1 

MATTMEQETCAHSLTFEECPKCSALQYRNGFYLLKYDEEWYPEELLTDGEDDVFDPELDMEVVFELQGNSTSSDKNNSSSEGNEGVIINNFYSNQYQNSI
DLSANAAGSDPPRLRSIFESLSGAVNAFSNMLPLLADQNTEEMENLSDRGLKTLPAIRSQTPSQQWAVLSVMVPFMMESIRHHVLTLLQKRFWRWKGTTP
SRLMIGHQHKSPLSTSAFPFLTSCPVKMVVSLVALRRHYLVKTGWRVQVQCNASQFHAGGLLVFMAPEYPTLDAFAMDNRWSKDNLPNGTRTQTNKKGPF
AMDHQNFWQWTLYPHQFLNLRTNTTVDLEVPYVNIAPTSSWTQHASWTLVIAVVAPLTYSTGASTSLDITASIQPVRPVFNGLRHETLSRQSPIPVTIRE
HAGTWYSTLPDSTVPIYGKTPVAPSNYMVGEYKDFLEIAQIPTFIGNKIPNAVPYIEASNTAVKTQPLATYQVTLSCSCLANTFLAALSRNFAQYRGSLV
YTFVFTGTAMMKGKFLIAYTPPGAGKPTSRDQAMQATYAIWDLGLNSSYSFTVPFISPTHFRMVGTDQVNITNADGWVTVWQLTPLTYPPGCPTSAKILT
MVSAGKDFSLKMPISPAPWSPQGVENAEKGVTENTNATADFVAQPVYLPENQTKVAFFYNRSSPIGAFTVKSGSLESGFAPFSNGTCPNSVILTPGPQFD
PAYDQLRPQRLTEIWGNGNEETSKVFPLKSKQDYSFCLFSPFVYYKCDLEVTLSPHTSGNHGLLVRWCPTGTPTKPTTQVLHEVSSLSEGRTPQVYSAGP
GISNQISFVVPYNSPLSVLSAVWYNGHKRFDNTGSLGIAPNSDFGTLFFAGTKPDIKFTVYLRYKNKRVFCPRPTVFFPWPTSGDKIDMTPRAGVLMLES
PNALDISRTYPTLHVLIQFNHRGLEVRLFRHGHFWAETRADVILRSKTKQVSFLSNGNYPSMDSRAPWNPWKNTYQAVLRAEPCRVTMDIYYKRVRPFRL
PLVQKEWPVREENVFGLYRIFNAHYAGYFADLLIHDIETNPGPFMFRPRKQVFQTQGAAVSSMAQTLLPNDLASKAMGSAFTALLDANEDAQKAMKIIKT
LSSLSDAWENVKETLNNPEFWKQLLSRCVQLIAGMTIAVMHPDPLTLLCLGTLTAAEITSQTSLCEEIAAKFKTIFITPPPRFPTISLFQQQSPLKQVND
IFSLAKNLDWAVKTVEKVVDWFGTWIVQEEKEQTLDQLLQRFPEHAKRISDLRNGMAAYVECKESFDFFEKLYNQAVKEKRTGIAAVCEKFRQKHDHATA
RCEPVVIVLRGDAGQGKSLSSQVIAQAVSKTIFGRQSVYSLPPDSDFFDGYENQFAAIMDDLGQNPDGSDFTTFCQMVSTTNFLPNMASLERKGTPFTSQ
LVVATTNLPEFRPVTIAHYPAVERRITFDYSVSAGPVCSKTEAGYKVLDVERAFRPTGEAPLPCFQNNCLFLEKAGLQFRDNRTKEIISLVDVIERAVAR
IERKKKVLTTVQTLVAQGPVDEVSFHSVVQQLKARQQATDEQLEELQEAFAKVQERNSVFSDWLKISAMLCAATLALSQVVKMAKAVKQMVKPDLVRVQL
DEQEQGPYNETARVKPKTLQLLDIQGPNPVMDFEKYVAKHVTAPIGFVYPTGVSTQTCLLVRGRTLVVNRHMAESDWTSIVVRGVTHARSTVKILAIAKA
GKETDVSFIRLSSGPLFRDNTSKFVKAGDVLPTGAAPVTGIMNTDIPMMYTGTFLKAGVSVPVETGQTFNHCIHYKANTRKGWCGSALLADLGGSKKILG
IHSAGSMGIAAASIVSQEMIRAVVNAFEPQGALERLPDGPRIHVPRKTALRPTVARQVFQPAYAPAVLSKFDPRTEADVDEVAFSKHTSNQESLPPVFRM
VAKEYANRVFTLLGKDNGRLTVKQALEGLEGMDPMDRNTSPGLPYTALGMRRTDVVDWESATLIPFAAERLRKMNEGDFSEVVYQTFLKDELRPIEKVQA
AKTRIVDVPPFEHCILGRQLLGKFASKFQTQPGLELGSAIGCDPDVHWTAFGVAMQGFERVYDVDYSNFDSTHSVAMFRLLAEEFFTPENGFDPLTREYL
ESLAISTHAFEEKRFLITGGLPSGCAATSMLNTIMNNIIIRAGLYLTYKNFEFDDVKVLSYGDDLLVATNYQLDFDKVRASLAKTGYKITPANTTSTFPL
NSTLEDVVFLKRKFKKEGPLYRPVMNREALEAMLSYYRPGTLSEKLTSITMLAVHSGKQEYDRLFAPFREVGVVVPSFESVEYRWRSLFW

♦Leader protein: promotes host NUP62, NUP153, and NUP214 phosphorylation and induces cessation of active nucleocytoplasmic transport. Proteins with NLS signals fail to import, cellular mRNAs fail to export, and some proteins small enough for diffusion are not retained anymore. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and to evade host immune response (By similarity).
♦ Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes. The capsid interacts with host VCAM1 to provide virion attachment on murine vascular endothelial cells (By similarity).
♦ Protein VP0: VP0 precursor is a component of immature procapsids.
♦ Protein 2B: Affects membrane integrity and cause an increase in membrane permeability.
♦ Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).
♦ Protein 3A, via its hydrophobic domain, serves as membrane anchor.
♦ Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. Cleaves host PABP1, this cleavage is important for viral replication.
♦ RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals.
♦ Protein 2A: is involved in host translation shutoff. Nuclear localization is required for this function (By similarity).

♦ Protein VP2: Virion. Host cytoplasm .
♦ Protein VP3: Virion. Host cytoplasm .
♦ Protein VP1: Virion. Host cytoplasm .
♦ Protein 2B: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 2C: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 3A: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 3B: Virion .
♦ Protease 3C: Host cytoplasm .
♦ RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane
♦ Peripheral membrane protein
♦ Cytoplasmic side . Note=Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity). .
♦ Protein 2A: Host nucleus .

♦DOMAIN 1279 1445 SF3 helicase.
♦ DOMAIN 2059 2177 RdRp catalytic.

♦ACT_SITE 1671 1671 For protease 3C activity.
♦ ACT_SITE 1703 1703 For protease 3C activity.
♦ ACT_SITE 1784 1784 For protease 3C activity.