Results | viHumans

UniProt ID

B3SRS2

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]

Gene Name

Not Available

Protein Name

Non-structural protein 2 (NSP2) (EC 3.6.4.-) (NCVP3) (Non-structural RNA-binding protein 35) (NS35)

Organism Name

Rotavirus A (strain RVA/Human/United States/D/1974/G1P1A[8]) (RV-A)

Taxonomic Lineage

Viruses> DsRNA Viruses> Reoviridae> Sedoreovirinae> Rotavirus> Rotavirus A> Rotavirus G1> Rotavirus A (strain RVA/Human/United States/D/1974/G1P1A[8]) (RV-A)

RefSeq

NC_011500.2 ; NC_011501.2 ; NC_011502.2 ; NC_011503.2 ; NC_011504.2 ; NC_011505.2 ; NC_011506.2 ;
NC_011507.2 ; NC_011508.2 ; NC_011509.2 ; NC_011510.2 ; NC_011511.2

PubMed ID

18786998

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							MAELACFCYPHLENDSYKFIPFNSLAIKCMLTAKVDKKDQDKFYNSIIYGIAPPPQFKKRYNTSDNSRGMNYETTMFNKVAILICEALNSIKVTQSDIAN
VLSRVVSVRHLENLVLRKENHQDVLFHSKELLLKSVLIAIGQSKEIETTATAEGGEIVFQNAAFTMWKLTYLDHKLMPILDQNFIEYKITLNEDKPISDV
CVKELVAELRWQYNRFAVITHGKGHYRVVKYSSVANHADRVFATYKNNTKSGNVTDFNLLDQRIIWQNWYAFTSSMKQGNTLDVCKKLLFQKIKQEKNPF
KGLSTDRKMDEVSHVGI
						

Length

317

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

02-09-2008

Protein Existence

Inferred from homology

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.

EC

3.6.4.-

Experimentally known GO terms

GO:0003723 ; GO:0005524 ; GO:0016817 ; GO:0019079 ; GO:0030430 ;
GO:0046872

Subcellular Location

Host cytoplasm . Note=Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging. .

Domain(s)

Not Available

Motif(s)

Not Available

Methods of 3D structure generation

Predicted/Modelled

Experimetally known structure

Not Available

Active Site(s)

ACT_SITE 225 225 For NTPase and RTPase activities.

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available