Results | viHumans

UniProt ID

P36719

Status

Reviewed

Host

Homo Sapiens (Human) [TaxID: 9606]

Gene Name

E1

Protein Name

Replication protein E1 (EC 3.6.4.12) (ATP-dependent helicase E1)

Organism Name

Human Papillomavirus Type 3

Taxonomic Lineage

Viruses> DsDNA Viruses> No RNA Stage> Papillomaviridae> Alphapapillomavirus> Alphapapillomavirus 2> Human Papillomavirus Type 3

RefSeq

Not Available

PubMed ID

8205838

Pathways Involved

Various pathway(s) in which protein is involved

KEGG

Not Available

Reactome

Not Available

Sequence

							MDDTSGTEGECSELERAGGWFMVEAIVDRRTGDTVSSDEDEEEDGGEDLVDFIDDRPVGDGQEVAQELLLQQAAADDDVEVQTVKRKFAPSPYFSPVCVH
PSIENELSPRLDAIKLGRQTSKAKRRLFELPDSGYGQTQVDTESGPKQVQDICKTSQQDGCQGADEGRGRNVGGNGSQEEERAGGDGEESQTESVQTDTT
ACGVLAILKASNHKATLLGKFKEQFGLGFNELIRHFKSNKTVCSDWVVCVFGVYCTLAESFKTLIQPQCEYAHIQVLSCQWGMTVLTLVRFKRAKNRETV
AKGFSTLLNVPENHMLIEPPKLRSAPAALYWFKTSLSNCSEVFGETPEWIVRQTVVGHALEEAQFSLSEMVQYAYDHDITDESTLAYEYALQADTDANAA
AFLASNCQAKYVKDACTMCRHYKRGEQARMNMSEWIKFRGDKIQGDGDWKPIVQYLRYQDVEFIPFLCALKSFLQGIPKKSCIVFYGPADTGKSYFCMSL
LKFLGGVVISYANSSSHFWLQPLAEAKIGLLDDATSQCWCYIDTYLRNALDGNQVCIDRKHRALLQLKCPPLLITTNINPLGDERWKYLRSRLQVFTFNN
KFPLTTQGEPLYTLNDQNWKSFFQRLWARLNLTDPEDEEDNGNTSEPFRCVPGQNTRTV
						

Length

659

Alternate Sequence

Not Available

Non-standard residue

Not Available

Date of last Sequence modification

01-06-1994

Protein Existence

Inferred from homology

Amino Acid composition

Amino Acid	Count	% Frequency	Amino Acid	Count	% Frequency
Alanine (A)			Leucine (L)
Arginine (R)			Lysine (K)
Asparagine (N)			Methionine (M)
Aspartic Acid (D)			Phenylalanine (F)
Cysteine (C)			Proline (P)
Glutamine (Q)			Serine (S)
Glutamic Acid (E)			Threonine (T)
Glycine (G)			Tryptophan (W)
Histidine (H)			Tyrosine (Y)
Isoleucine (I)			Valine (V)

Sum of Absolute Deviation from Amino Acid Occurrence Frequencies

Aliphatic Index

Instability Index

Secondary Structure features

% Number of Residues in Helices	% Number of Residues in Strands	% Number of Residues in Coils

Structural Difficulty(SD) index

Role(s)

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.

EC

3.6.4.12

Experimentally known GO terms

GO:0003677 ; GO:0004003 ; GO:0005524 ; GO:0006260 ; GO:0042025

Subcellular Location

Host nucleus .

Domain(s)

DOMAIN 461 611 SF3 helicase.

Motif(s)

MOTIF 85 87 Nuclear localization signal. ; MOTIF 107 116 Nuclear export signal.

Methods of 3D structure generation

Predicted/Modelled

Experimetally known structure

Not Available

Active Site(s)

Not Available

Predicted 3D Structures

Protein couldn't be modeled using I-Tasser and Raptor X because of length constraints of the software.

Experimentally known Ligand(s)

Not Available

Virtually Screened Ligands

Virtual screening has been performed using RASPD

						Million Molecules

					
Best 20 Hit molecules 
Not Available